Expressed Sequence Tags (ESTs) are short DNA sequences, usually based on a single dideoxy sequencing run, representing sequences expressed in an organism under particular conditions. Many EST libraries have been constructed containing huge numbers of clones, from organisms such as yeast, humans, and Drosophila.
We have recently started a small-scale EST project. We had previously prepared cDNA libraries from S. commune mycelia grown on minimal medium (MIN), or transferred to low-nitrogen medium and harvested six hours (6HR) or twelve hours (M01) after transfer.
Students in our lab have selected a large number of clones from each library. Each clone is excised from the lambda-Zap vector (Stratagene) to generate a pBluescript phagemid clone. These phagemids are isolated and sequenced.
The sequences have been submitted to dbEST, a part of the GenBank database. Students on the project included Kim Foster, Erin Jackson, and Sebastian Guettler, project managers; Jennifer Abner, Adelaide Beiser, Sam Buchanan, Tameka DeVoil, Martha Huck, Loren Honaas, Curtis Jansen, Scott Luchese, Isaac Powell, and Yang Tian. The project was supported by grants from the Southeast Missouri St. U. GRFC (Grants and Research Funding Committee).
See also
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Guettler S, Jackson EN, Lucchese SA, Honaas L, Green A*, Hittinger CT, Lilly WL, and Gathman AC. 2003. ESTs from the basidiomycete Schizophyllum commune grown on nitrogen-replete and nitrogen-limited media. Fungal Genetics and Biology 39:191-198. |